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Mutation of the aromatic residues in Bnd4 alters its product profile. HPLC analyses of the Bnd4 variants in comparison with native Bnd4 (A–C) and the cembrene synthase DtcycA (D). Control reactions are no enzyme negative controls. (E) Structures of identified diterpene products. Enzyme products labeled with asterisks (*) were uncharacterized.
