Fig. 1.

HIIT improves physical performance in aged female mice. The experimental timeline begins at 21-months of age in female C57BL/6 J mice (A). At 24-months of age, mice were administered HIIT (n = 14) or remained sedentary (SED, n = 11) for 8 weeks. Body weight was measured weekly (A) and the change in body weight was assessed from the start to the end of the HIIT phase (B). Baseline uphill interval treadmill (C) was used to assign the initial intensity for HIIT exercise and as a marker for subsequent impacts of the exercise. A “*” indicates p < 0.05 in an unpaired t-test between groups, a “#” and a “##” indicates p < 0.05 and p < 0.01, respectively in a paired t-test within groups, “B” indicates baseline, and “E” indicates endpoint.