FIGURE 1.

Summary of inputs and outputs of RESTRICT assay. (a) RESTRICT uses nucleic acid templates, nucleotides, recombinant HIV‐1 reverse transcriptase, primers, and intercalating dyes to measure the concentration of nucleotide analogs. The nucleic acid templates have primer‐binding domains to initiate DNA synthesis and chain terminating domains designed to bind specific nucleotide analogs. (b) The key contributions to fluorescence output of the RESTRICT assay come from (i) full‐length dsDNA products, (ii) fragment dsDNA products, and (iii) unpolymerized ssDNA template. (c) Typical sigmoidal fluorescence versus concentration profile obtained from RESTRICT assays illustrating the high, intermediate, and low fluorescence obtained from the different assay end products