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Detection of TFV‐DP and 3TC‐TP using DNA templates designed for selective detection of each drug without cross‐reactivity. TFV‐DP (A analog) was detected with a thymidine‐rich TTCA template that excluded G bases to prevent cross‐reactivity with 3TC‐TP (C analog). Similarly, 3TC‐TP was detected with a guanosine‐rich GGCA template that excluded T bases. N = 3
