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. 2022 Aug 23;30(1):22–37. doi: 10.1038/s41417-022-00515-9

Fig. 3. CDK5RAP3 in gastric cancer cells induces macrophages to differentiate into M1 polarized macrophages in vitro.

Fig. 3

A Schematic diagram showing the experimental process of coculture of gastric cancer cells and macrophages. B Typical bright-field images of macrophages processed by each coculture system are shown. (Magnification, ×200). Scale bar = 100 μm. C Flow cytometry was used to detect the expression of CD206 on the surface of differentiated macrophages. *P < 0.05; **P < 0.01, compared with Vector. D Flow cytometry was used to detect the expression of CD86 on the surface of differentiated macrophages. *P < 0.05; **P < 0.01, compared with Vector. E Under the method shown in A, the gene expression of TNF, INOS, IL1B, ARG1, TGFB1 and IL6 in monocytes was detected. *P < 0.05; **P < 0.01, compared with Lenti-emp. F Under the method shown in A, the gene expression of TNF, INOS, IL1B, ARG1, TGFB1 and IL6 in monocytes was detected. *P < 0.05; **P < 0.01, compared with Lenti-scr.