Skip to main content
. 2022 Aug 23;30(1):22–37. doi: 10.1038/s41417-022-00515-9

Fig. 5. CDK5RAP3 negatively regulates the production of IL4 and IL10 by gastric cancer cells by inhibiting NF-κB nuclear transcription.

Fig. 5

A, B The expression of p-NF-κB p65 and NF-κB p65 in stably transfected BGC-823 cells was detected using Western blotting. C Immunofluorescence (IF) staining showed the localization of NF-κB p65 (red) and DAPI (blue) in stably transfected BGC-823 cells. Scale bar = 50 μm. D Flow cytometry was used to detect the expression of CD206 and CD86 on the surface of differentiated macrophages. **P < 0.01, compared with Vector. E, F Relative gene expression of IL4 and IL10 in stably transfected BGC-823 cells was determined by RT-PCR. G IL4 and IL10 expression in the culture supernatant of transfected BGC-823 cells was determined by ELISA. H, I Flow cytometry was used to detect the expression of CD206 and CD86 on the surface of differentiated macrophages. **P < 0.01, compared with Vector.