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. 2022 Aug 23;30(1):22–37. doi: 10.1038/s41417-022-00515-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A MTT assay and colony formation assay of BGC-823 cells cocultured with macrophages or single cultures. Days = 8. B Transwell assays of stably transfected BGC-823 cells were performed, and the quantification of the results is presented. Days = 8. C Western blotting were used to determine MMP2 expression of macrophages in different coculture systems. Coculture time = 48 h. D ELISA were used to determine MMP2 secretion by macrophages. E Relative gene expression of MMP2 expression in macrophages was determined by RT-PCR. F ELISA were used to determine MMP2 secretion by macrophages at different cocultivation time points. G MTT assay of BGC-823 cells in different treatment coculture systems. H, I Colony formation assay of BGC-823 cells in different treatment coculture systems. Days = 8. J Western blotting was used to detect the expression of E-cadherin, N-cadherin, vimentin and Snail in gastric cancer in different treatment coculture systems. K Relative gene expression of E-cadherin, N-cadherin, vimentin and Snail in gastric cancer tissues was determined by RT-PCR. *P < 0.05; **P < 0.01.