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Left: plots of 1H NMR spectra of (a) acyclic peptide 3 and (b) stapled peptide 4 with box highlighting the key signals of alkyne and triazole protons. Right: plots of 1H NMR spectra of (c) acyclic peptide 5 and (b) stapled peptide 6 with box highlighting the key signals of terminal alkenes and internal double bond protons. Spectra were recorded at 400 MHz in D2O.
