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. 2023 Jan 16;14:235. doi: 10.1038/s41467-022-35705-4

Fig. 2. Activation of mTORC1 is insufficient for α cell proliferation.

Fig. 2

a Representative images of α cells in control, TgαCA-MTOR (Tg(gcga:MTORL1460P, cryaa:tagRFP)), TgαCA-Rheb (Tg(gcga:RhebS16H, cryaa:tagYFP)), and gcgrDKO (gcgra−/−;gcgra−/−) larvae at 5 dpf (scale bar, 10 μm). b Quantification of α cell number in control, TgαCA-MTOR, TgαCA-Rheb and gcgrDKO larvae at 5 dpf (data represent the means ± SD, n = 15 for each group). c Quantification of α cell size in control, TgαCA-MTOR, TgαCA-Rheb and gcgrDKO larvae at 5 dpf (Data represent means ± SD, n = 16 for each group). d Representative images of EdU staining in control, TgαCA-MTOR and TgαCA-Rheb larvae at 5 dpf. EdU (red)-positive α cells are indicated by arrows (scale bar, 10 μm). e Quantification of EdU-positive α cell numbers in control, TgαCA-MTOR and TgαCA-Rheb larvae at 5 dpf (data represent the means ± SD, n = 9 for each group). ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey’s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a Source Data file.