Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 16;14:241. doi: 10.1038/s41467-023-35940-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a–c The frequency of occurrence of spontaneous resistance mutations is compared to the frequency of occurrence of resistant variants in the saturation editing libraries. This was done by plating either a wild-type culture or the different libraries onto medium containing different concentrations of the compound, i.e. 4x MIC (a), 8× MIC (b) or 32× MIC (c), and counting the number of colonies that developed after overnight growth. These numbers were normalized to the total cell numbers present in the wild-type culture or the libraries, respectively. d The location of targeted residues in the FabZ protein is shown for both CHIR-090 and PF-04753299. Residues are colored according to the number of times they were targeted in isolated resistant variants. Only one dimer of the FabZ hexamer is shown for clarity. Mutations are indicated in both chain A and B. e The location of targeted residues in the LpxC protein is shown for both CHIR-090 and PF-04753299. Residues are colored according to the number of times they were targeted in isolated resistant variants. The number of unique mutations in fabZ and lpxC that provide resistance to CHIR-090 or PF-04753299 and the number of mutations in murA that provide resistance to fosfomycin are shown, either grouped per library and compound (f) or grouped per compound only (g). For each library-compound combination, 35 resistant variants were isolated and their fabZ, lpxC or murA gene was sequenced. The mutations found are subdivided into categories based on the minimal number of SNPs necessary to provide the observed amino acid change. Source data are provided as a Source Data file. Fosf fosfomycin, ACP acyl carrier protein.