Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 17;9:6. doi: 10.1038/s41421-022-00490-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Schematic diagram showing the experimental procedure of AEBP1 knockdown. Three independent experiments were performed for each group. Normal human ventricular cardiac fibroblasts (passage 5-7) were used. b Bright-field images showing the cardiac fibroblasts after 48 h of transfection. Scale bar: 200 μm. c Phalloidin staining of actin filaments showing the morphological changes of cardiac fibroblasts caused by AEBP1 knockdown. Scale bar: 50 μm. d Ki67 staining showing that AEBP1 knockdown reduced the proliferation of cardiac fibroblasts. In the bar plot, each value represents the mean number of Ki67-positive cells across five representative fields of view. Scale bar: 20 μm. ^**P-value < 0.01, Student’s t-test. e mRNA expression of AEBP1 and markers for fibroblast activation in both groups. The expression level is represented as tags per million reads. f Network plot showing the functional enrichment of the upregulated genes in AEBP1-siRNA versus scrambled siRNA. g Network plot showing the functional enrichment of the downregulated genes in AEBP1-siRNA versus scrambled siRNA. In f and g, each node denotes an over-represented Reactome pathway, and the node size reflects the statistical significance. Functionally associated terms were in the same color. The enrichment significant threshold was set to an adjusted P-value < 0.05. h Western blot assay showing the changes in protein expression of AEBP1 and representative fibroblast activation markers by the knockdown of AEBP1. i Immunofluorescence staining of cultured fibroblasts showing an increased protein expression level of Collagen-I by the knockdown of AEBP1. Scale bar: 50 μm. j Quantitative analysis of the western blot and staining results. For bar plots, the data are presented as means±standard error of the mean (SEM). For the staining results, each value represents the mean fluorescence intensity across five representative fields of view. ^*P-value < 0.05, ^**P-value < 0.01, ^***P-value < 0.001. Student’s t-test.