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. 2023 Jan 16;13:854. doi: 10.1038/s41598-023-27859-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histological and histochemical characterization in Gp130 ecKO uterus before embryo implantation. (a) Representative images of uterine cross section of pre-implantation site on D4 (1600 h) from control and Gp130 ecKO. The depth of the uterine chamber (M-AM distance) is comparable between control and Gp130 ecKO (b, upper panel), and embryos are located in the deep antimesometrial side of the uterine chamber in both groups (b, lower panel). (c) Cell proliferation status detected by immunostaining of MKI67 at D1 (1000 h) and D4 (1600 h). (d) Immunostaining of phosphorylated STAT3 (pSTAT3) in pre-implantation uterus (D4, 1600 h). pSTAT3 is not detectable in the uterine epithelium from Gp130 ecKO. Scale bar, 100 µm (a,c, and d with lower magnification) and 50 µm (d with higher magnification). le luminal epithelium, ge glandular epithelium, st stroma, em embryo, M mesometrial side, AM antimesometrial side.