Table 3.

Folding thermodynamics of scFvs in the absence or presence of antigen

	Td (°C)	∆Td (°C)	∆Hcal (kJ mol−1)
(Trp33H/Tyr95H)
N1G9 scFva	66.2		395
N1G9 scFv + NPa	68.8	2.6	452
(Leu33H/Tyr95H)
N1G9_W33L scFva	64.0		418
N1G9_W33L scFv + NPa	69.8	5.8	493
(Leu33H/Gly95H)
C6_W33L scFva	47.4		405
C6_W33L scFv + NPa	60.2	12.8	459
(Trp33H/Gly95H)
9TG scFvb	68.4		567
9TG scFv + NPb	70.7	2.3	666
9T7 scFvb	52.7		402
9T7 scFv + NPb	62.5	9.8	644
C6 scFva	48.5		384
C6 scFv + NPa	67.0	18.5	585
E11 scFvb	50.1		570
E11 scFv + NPb	70.1	20.0	795

+ NP; molar ratio of scFv and NP is 1:10

∆T_d = T_d in the presence of NP − T_d in the absence of NP

^aData were taken from Sato et al. (2017)

^bData were taken from Nishiguchi et al. (2022)