FIG. 1.

Alignment of the deduced amino acid sequence of ALO from C. albicans (Ca-ALO) with those of other enzymes with similar activity: ALO from S. cerevisiae (10) (Sc-ALO), l-gulono-1,4-lactone oxidase from rat (17) (Rn-GLO), and l-galactono-1,4-lactone dehydrogenase from cauliflower (31) (Bo-GLD). Numbers on the right are amino acid positions. The regions where the sequences have been extended to allow optimal sequence alignment are indicated with dashes. Identical residues are shaded. The asterisk indicates the histidine residue believed to be responsible for covalent attachment of FAD. A 17-residue putative transmembrane segment predicted according to the method by Kyte and Doolittle (18) is underlined.