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. 2001 Jun;69(6):3939–3946. doi: 10.1128/IAI.69.6.3939-3946.2001

FIG. 2.

FIG. 2

Disruption of the ALO1 gene in C. albicans. (A) Restriction map of the ALO1 locus and insertion of the hisG-URA3-hisG cassette at the KpnI sites in ALO1 coding sequence. Endonuclease restriction sites: C, ClaI; E, EcoRI; H, HindIII; Hp, HpaI; K, KpnI; N, NcoI; S, SpeI. (B) Southern blot analysis with the sequence bracketed in panel A used as a probe. The DNA digested with HindIII was from the following strains: CAI4 ALO1/ALO1 (lane 1), WH201 Δalo1::hisG-URA3-hisG/ALO1 (lane 2), WH202 Δalo1::hisG/ALO1 (lane 3), WH203 Δalo1::hisG/Δalo1::hisG-URA3-hisG (lane 4), WH204 Δalo1::hisG/Δalo1::hisG (lane 5), and WH207 Δalo1::hisG/Δalo1::hisG::ALO1::URA3 (lane 6).