TABLE 2.
Activity of ALO and amount of EASC in C. albicans strains of different genetic backgrounda
| Strain | Activity of ALO (mU · mg of protein−1) | Amt of EASC (nmol · g [wet wt] of cells−1) |
|---|---|---|
| SC5314 (ALO1/ALO1) | 37.3 ± 5.1 | 427 ± 35 |
| WH203 (alo1/alo1) | <0.1b | <0.1c |
| WH205 (CAI4 carrying pRC2312) | 34.8 ± 4.2 | 336 ± 15 |
| WH206 (CAI4 carrying pWK203) | 119.0 ± 7.3 | 1,330 ± 52 |
| WH207 (ALO1 reintegrant) | 19.4 ± 3.8 | 237 ± 26 |
a
Activity of ALO and amount of EASC in C. albicans were measured from exponentially growing cells in minimal defined medium. Values are means ± standard errors (n = 3).
b
Lower limit for assay of ALO activity.
c
Lower limit of detection for EASC.