FIGURE 6.

Effect of BCAM on spheroid dispersion. (A) Spheroids of BCAM‐overexpressing OVCAR8 clones (BCAM1‐2, BCAM2‐1) and cells transduced with the empty vector (pcDNA‐3) were embedded in a 3D collagen matrix for 48 h. The photomicrographs show a clear dispersion only for the BCAM‐overexpressing cells. Scale bar: 500 μm. (B) Quantification of dispersion of spheroids in panel A plus one additional clone for each condition (n = 3 biological replicates each). *p < .05; **p < .01; ***p < .001; ns: not significant by unpaired t‐test. (C) Spheroids were generated from BCAM‐overexpressing BCAM1‐2 cells in the presence or absence of exogenous LN‐511 (10 μg/ml) as in panel A. Pictures were taken at times 0 and 48 h after embedding. (D) Quantification of dispersion of spheroids generated from four different BCAM‐overexpressing and four control clones in the presence or absence of exogenous LN‐511 as in panel C (n = 3 biological replicates each). **p < .01 by paired t‐test