TABLE 2.
Summary of immunohistochemical analysis of BCAM, LAMA5, COL1 and COL4 in matched samples of OC metastases (Met) and spheroids from ascites (Asc). Staining intensities were classified as follows: Negative (0, black), weak (1, blue), moderate (2, brown) and strong (3, red). Metastatic sites analysed are listed in Table S2. Examples are shown in Figures 3 and S13
|
Patient ID |
BCAM | Laminin α5 (LAMA5) | Collagen I (COL1) | Collagen IV (COL4) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Met tumour | Met stroma | Asc spheroid | Met tumour | Met stroma | Asc spheroid | Met tumour | Met stroma | Asc spheroid | Met tumour | Met stroma | Asc spheroid | |
| OC26 | 3 | 2 | 3 | 0 | 0 | 3 | 1 | 2 | 3 | 0 | 1 | 0 |
| OC27 | 3 | 2 | 3 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 |
| OC54 | 1 | 1 | 2 | 0 | 0 | 2 | 1 | 2 | 2 | 0 | 1 | 0 |
| OC66 | 1 | 1 | 3 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 1 | 0 |
| OC67 | 2 | 2 | 3 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 1 | 0 |
| OC84 | 3 | 2 | 3 | 3 | 0 | 2 | 1 | 3 | 2 | 0 | 1 | 1 |
| OC114* | 1 | 1 | 3 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 1 | 0 |
| OC122 | 2 | 2 | 3 | 2 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 |
*Small metastases from patient OC114 were positive of LAMA5 (score 2; see Figure 3).