Figure 3.

Overexpression of miR-143-3p inhibits the HG-induced inflammatory response in MIN6 cells. MIN6 cells were transfected with miR-143-3p mimics or mimics NC for 24 h, before they were cultured in 16.7 mM HG for additional 24 h. The levels of (A) TNF-α, (B) IL-6 and (C) IL-8 were then measured by ELISA. Data are presented as the means ± SD from three individual experiments. ^*P<0.05 and ^**P<0.01 vs. Control; ^##P<0.01 vs. HG + mimics NC group. miR, microRNA; HG, high glucose; NC, negative control.