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SDS-PAGE and immunoblot analysis of DbpA expression in B. burgdorferi 297 cultivated at either 23°C (2 × 107 spirochetes) or 34°C (107 spirochetes). Polyclonal rat anti-DbpA antiserum and monoclonal antibody 8H3-33, directed against FlaB, were used as the respective antibody probes. Numbers at the left denote protein apparent molecular weights (in thousands).
