FIG. 3.

IFAs of borreliae harvested from the salivary glands of B. burgdorferi-infected I. scapularis nymphs. Nymphs were allowed to feed on normal C3H/HeJ mice for 48 to 72 h, at which time the ticks were removed. Tick salivary glands were then dissected out (with caution to exclude midgut contents) and homogenized (by repeated gentle pipeting) in 10-μl aliquots of phosphate-buffered saline. The samples were then divided into two equal portions on microscope slides, dried, fixed with acetone, and probed with rat antiserum directed against either a whole-cell lysate of B. burgdorferi 297 (A) or DbpA (B). All other procedures were as described for Fig. 2. The panels shown are representative of at least 30 microscope fields examined in each of five separate salivary gland smears probed with each antiserum.