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. 2023 Jan 17;133(2):e164501. doi: 10.1172/JCI164501

Figure 4. Identification of bin 1 and 3 epitopes specific to sustained responders in OIT.

Figure 4

(A) Protein sequence of Ara h 2.0201 with shading conformational residues identified by x-ray crystallography on Ara h 2.0101 (13T1 in teal, 22S1 in red), chimeric proteins (bin 2 in orange), sequential epitopes (underlined), and helices (bracketed). (B) Epitope binning with primary bin 1 or 3 conformational antibodies and secondary antibodies recognizing sequential epitopes. (C) Biolayer sensorgrams using primary antibodies (top) recognizing sequential epitopes before binding of secondary bin 1 conformational mAbs (left) identified two bin 1 overlapping epitopes (colored boxes). (D) Response rates of secondary conformational bin 1 antibodies after binding of primary DRRCQSQLER and QGRQQEQQ antibodies. (E and F) Ribbon representations of overlapping epitopes 1.1 and 1.2 (blue) and bin 3 (red) with sequential epitopes (bracketed) overlaid with surface representation.