Skip to main content
. 2023 Jan 17;133(2):e153470. doi: 10.1172/JCI153470

Figure 3. miR-34a restricts CD47 activity and restores the phagocytic function of macrophages in vitro.

Figure 3

(A) Scatter plot comparison illustrating miRNAs that were differentially expressed between malignant and normal lung tissues from KrasLSL-G12D/+ mice. Down, downregulated; Up, upregulated. (B) Dendrogram generated by unsupervised hierarchical cluster analysis showing the separation of tumors from normal tissues based on miRNA profiling (10 upregulated vs. 40 downregulated). (C) Quantitative RT-PCR analysis of the 9 most changed miRNAs in A (n = 5 mice). (D and E) Effect of the miR-34a mimic or the miR-34a antisense strand on CD47 expression in H358 cells. (F) Effect of the miR-34a mimic on macrophage phagocytosis of H358 cells. Cells were transfected with the miR-34a mimic and/or a CD47-expressing plasmid. After 48 hours, cells were subjected to a macrophage phagocytosis assay. Representative FACS images and quantification results (n = 5) are shown. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, by unpaired t test (C) or 1-way ANOVA (F).