Fig. 1.

Nectin-4 is differentially expressed at the gene and protein levels in invasive PSCC. (A) Waterfall plots demonstrating variability in Nectin-4 gene expression in internal (left panel) and external (right panel; GSE57955) tumor cohorts; the external cohort gene expression is relative to normal glans tissue. The dashed line indicates 25th quantile of global gene expression. (B) Representative image of subsetted TMA cores stained for Nectin-4 protein with an enlarged view of a single core (left panel) and histogram demonstrating IHC scoring (cell counts positive and intensity of stain) stratified by HPV infection status and normal penile tissue (right panel). (C) Boxplots comparing Nectin-4 gene (left panel) and protein (right panel) expression based on HPV infection and normal penile tissue. HPV = human papillomavirus; IHC = immunohistochemical; PSCC = penile squamous cell carcinoma; TMA = tissue microarray. * p < 0.05. *** p < 0.001.