Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 12;11(1):e006136. doi: 10.1136/jitc-2022-006136

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Analysis of pretreatment and on-treatment liver lesion biopsies of a PR mCRC participant (Pt 059) by fluorescence multiplex microscopy. The on-treatment lesion was biopsied 5 weeks after treatment commenced. Representative areas of pretreatment (A) and on-treatment (B) lesions are shown with the regions of tumor tissue indicated in red (C, D). In this example the markers CD3 (yellow), CD4 (cyan), CD8 (green), Ki67 (red) and nuclei are stained with DAPI (blue). To exemplify CD8 staining of these biopsy regions, three-dimensional presentation of the CD8 label fluorescence intensity is presented (E, F). Identification of positively staining cells in the tumor regions of the biopsies was performed for various markers, including CD4⁺ cells (G), CD8⁺ cells (H) and CD11c⁺ cells (I) which were all elevated in the on-treatment biopsy compared with the prebiopsy. The CD4 and CD8 data were collected from two separately stained sections (of each biopsy) allowing the data to be averaged and errors bars included (±SE). mCRC, metastatic colorectal cancer; PR, partial response.