Table 1.

Detailed staining conditions and order of the Opal™-TSA mIHC 7-marker panel for human FFPE tissue sections

Primary antibody	Antibody dilution (final concentration)	Antibody incubation time	Opal™ fluorophore (dilution)
Anti-human CD21 [1F8]	1:10 (199 μg/mL)	Overnight (16–18 h)	520 (1:100)
Anti-human CD208/DC-LAMP	1:100 (10 μg/mL)	1 h	540 (1:100)
Anti-human CD4 [EPR6855]	1:1000 (0.143 μg/mL)	1 h	570 (1:100)
Anti-human PNAd [MECA-79]	1:100 (10 μg/mL)	Overnight (16–18 h)	620 (1:100)
Anti-human CD8 alpha [C8/144B]	1:50 (10 μg/mL)	1 h	650 (1:100)
Anti-human CD19 [EPR5906]	1:300 (6.12 μg/mL)	1 h	690 (1:100)