Table 2.

Detailed staining conditions and order of the Opal™-TSA mIHC 6-marker panel for formalin free zinc salt-fixed tissue sections

Primary antibody	Antibody dilution	Antibody incubation time	Opal™ fluorophore (dilution)
Anti-mouse PNAd antibody [MECA-79]	1:5000	1 h	690 (1:150)
Anti-mouse CD4 antibody [4SM95]	1:1000	Overnight (16–18 h)	620 (1:150)
Anti-mouse CD8 antibody [4SM15]	1:2000	1 h	570 (1:150)
Anti-mouse CD19 antibody	1:200	Overnight (16–18 h)	650 (1:150)
Anti-mouse CD21 antibody [EP3093]	1:1500	1 h	540 (1:150)