Zinc fixative
| Reagent | Final concentration | Amount |
|---|---|---|
| TRIS base | 99.9 mM | 12.1 g |
| Calcium acetate | 2.84 mM | 0.5 g |
| Zinc acetate | 22.8 mM | 5.0 g |
| Zinc chloride | 36.7 mM | 5.0 g |
| ddH2O | N/A | 1.0 L |
| Total | N/A | 1.0 L |
Dissolve 12.1 g of Tris base in 1 L of dH2O using a magnetic stirrer and adjust the pH to 7.4. Add 0.5 g of calcium acetate, 5 g of zinc acetate and 5 g of zinc chloride. The final pH will be approximately 6.5–7.0, do not readjust pH as this may cause the zinc to precipitate. Store at room temperature (RT, 15°C–30°C), for a maximum of 24 months.