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10× TBST
| Reagent | Final concentration | Amount |
|---|---|---|
| TRIS base | 0.2 M | 24.3 g |
| Sodium chloride | 1.5 M | 87.7 g |
| ddH2O | N/A | 1.0 L |
| Total | 10× | 1.0 L |
Dissolve 24.3 g of Tris base with 87.7 g of Sodium chloride in 1 L of dH2O using a magnetic stirrer. Store at RT (15°C–25°C), for a maximum of 24 months.
