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Tris-EDTA buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| TRIS base | 99.9 mM | 12.1 g |
| EDTA | 12.7 mM | 3.7 g |
| ddH2O | N/A | 1.0 L |
| Total | N/A | 1.0 L |
Dissolve 12.1 g of Tris and 3.7 g of EDTA in 1 L of dH2O using a magnetic stirrer and adjust the pH to 9.0. Store at RT (15°C–25°C), for a maximum of 3 months.
