Fig. 5.

Gabapentin partly reverses decreased sniffing behaviors in Fabry rats. (A) Percentage change in KO animals relative to WT for exploratory, self-care, and locomotion related behaviors. Green box represents the behavior with the greatest consistent change in both male and female rats. Fraction of the dark phase spent sniffing for female (B) or male (C) rats. (D) Whole-body plethysmography of female WT or KO rats. Sniffing (breaths > 250 BPM) were recorded continuously over three days and average sniffing time/hour calculated. (E) Male rats were given i.p. saline injections for 2 days followed by gabapentin (100 mg/kg) injections for 2 days. Fraction of the dark phase spent sniffing was calculated over 4 h following injection. Sniffing data was averaged over 2 days. (F) Heatmap of exploratory, self-care, and locomotion related behaviors after saline or gabapentin injection. Green boxes indicate behaviors that were changed after gabapentin administration towards WT levels. Behaviors marked with “&” are > 200 % increase/decrease due to low WT saline average for that behavior. (A-C): N = 10 wt-Male, 10 KO-Male, 11 wt-Female, 10 Het-Female, and 9 KO-Female. (D): N = 6 wt-Females, 7-KO Females, (E-F): N = 8 wt-Males, 10 KO-Males. *p < 0.05.