Lysis buffer I

Reagent	Stock concentration	Final concentration	Amount (μL)
Tris-HCl pH 8.0	1 M	50 mM	75
NaCl	5 M	150 mM	45
Triton X-100	20%	1%	75
Sodium deoxycholate	10%	0.5%	75
SDS	20%	0.1%	7.5
MgCl2	1 M	1 mM	1.5
Benzonase nuclease	250 U/μL	100 mU/μL	0.6
Halt Protease Inhibitors	100×	1×	15
MilliQ water	N/A	N/A	1205.4
Total	N/A	N/A	1500