| Reagent | Stock concentration | Final concentration | Volume (μL) |
|---|---|---|---|
| 10× Buffer | 10× | 1× | 2.5 |
| MnCl2 | 100 mM | 10 mM | 2.5 |
| UDP-sugar | 10× | 1× | 2.5 |
| Peptide | 1 mM | 250 μM | 6.25 |
| WT/BH GalNAc-T | 10× | 1× | 2.5 |
| MilliQ water | N/A | N/A | 8.75 |
| Total | N/A | N/A | 25 |
| Reagent | Stock concentration | Final concentration | Volume (μL) |
|---|---|---|---|
| 10× Buffer | 10× | 1× | 2.5 |
| MnCl2 | 100 mM | 10 mM | 2.5 |
| UDP-sugar | 10× | 1× | 2.5 |
| Peptide | 1 mM | 250 μM | 6.25 |
| WT/BH GalNAc-T | 10× | 1× | 2.5 |
| MilliQ water | N/A | N/A | 8.75 |
| Total | N/A | N/A | 25 |