Mixed infections of C3H/HeOuJ mice. Two strains were mixed in equal numbers and inoculated into female mice. Mice received 2.0 × 108 CFU and were sacrificed 2 days after infection. (A) Mixed infections comparing CP9 (CNF1+ Lac+) with CP9lacZ (CNF1+ Lac−). Eleven female C3H/HeOuJ mice were inoculated. Symbols: ▵, CP9; ▴, CP9lacZ. (B) Mixed infections comparing CP9lacZ with CP9cnf1. Twenty female C3H/HeOuJ mice were inoculated. Counts of CP9lacZ were significantly higher than those of CP9cnf1 in the urine, bladders, and kidneys (Student's t test, P < 0.05). Symbols: ▵, CP9lacZ, ▴, CP9cnf1. Points represent bacterial numbers in individual samples, and bars represent the means of those samples.