Figure 2.

M2-TAMs significantly promoted the proliferation of LUAD cells, both in vitro and in vivo. (A) Bright field images of Thp-1 cells by microscopy showed that Thp1 cells were induced to M2-TAMs by PMA, IL-4, and IL-6 after recovery. (B) Immunofluorescence was used to detect the induction of M2-TAMs (CD206+). (C) CCK8 assay detected the proliferation level of LUAD cells A549 and H1975 in M2-TAMs co-culture group or control group, n=3. (D) Colony formation assay and crystal violet staining of LUAD cells A549 and H1975 in M2-TAMs co-culture group or control group, n=3. (E) Assay of tumorigenicity in nude mice. A549 (3×10⁶) cells in the co-culture or control group were subcutaneously inoculated into the murine left flank, n=5. (F) Volume of subcutaneous tumors in mice, n=5. (G) Weight of subcutaneous tumors in mice on day 30, n=5. Data are presented as mean ± SEM, *P<0.05, **P<0.01, and ***P<0.001 according to two-tailed independent t-test. M2-TAMs,M2 phenotype tumor-associated macrophages; LUAD, lung adenocarcinoma.