Figure 6.

Mis-glycosylated PSG5 is present in the placenta and maternal circulation in patients who develop ePE

(A) PSGs detected in maternal serum by quantitative TMT-LC-MS/MS analysis. Gestational age-matched albumin-depleted normotensive control (NC) and ePE serum samples were purified by WGA affinity chromatography before mass spectrometry. Levels of PSGs are plotted as a median with 95% Cl, n = 4-5. Mann Whitney U test.

(B) PSG5 level and glycosylation profile in NC and ePE serum. Western blot band intensities corresponding to “More complex glycans” and “Less complex glycans” were quantified separately and are plotted together with “Total” which is the overall band intensity. Data are mean ± SD, n = 9-10. Unpaired t-test.

(C) The ratio of WGA affinity-purified to total PSG5 in normotensive term control (NTC) and ePE placentas. The results in B-C are expressed as mean ± SD, n = 7-10. Unpaired t-test.

(D) Sialyltransferases involved in N-glycan sialylation are down-regulated in repetitive hypoxia-reoxygenation (rHR)-treated BeWo cells. Cells were challenged with rHR (a 6 h cyclic pattern between 20% and 1% O₂ concentration for 24 h). The number of normalized counts from RNA-Seq are plotted. Data are mean ± SD, n = 5. Paired t-test. ∗∗∗∗ indicates p < 0.0001.