Table 1.

Predicted impact of receptor-binding motif variations in the SARS-CoV-2 variant B.1.1.529 (Omicron, sublineage: BA.1) on interactions with the host.^a

ACE2b binding site mutations	ACE2 binding (Δlog10 KD appc,d)	Protein expression (Δlog mean MFIe,f)	ACE2 contact with SARS-CoV-2	RSAg bound	SARS-CoV-1 amino acid	RaTG13 amino acid	GD Pangolin-CoV amino acid
G339D	0.06	0.30	false	0.47	G	G	G
S371L	–0.14	–0.61	false	0.46	S	S	S
S373P	–0.08	–0.22	false	0.48	F	S	S
S375F	–0.55	–1.81	false	0.48	S	S	S
K417N	–0.45	0.10	true	0.19	V	K	R
N440K	0.07	–0.12	false	0.68	N	H	N
G446S	–0.20	–0.40	true	0.55	T	G	G
S477N	0.06	0.06	false	0.76	G	S	S
T478K	0.02	0.02	false	0.48	K	K	T
E484A	–0.07	–0.23	false	0.50	P	T	E
Q493R	–0.09	–0.06	true	0.10	N	Y	Q
G496S	–0.63	0.12	true	0.04	G	G	G
Q498R	–0.06	0.10	true	0.00	Y	Y	H
N501Y	0.24	–0.14	true	0.03	T	D	N
Y505H	–0.71	0.16	true	0.12	Y	H	Y

^aBased on the study of Starr et al [21].

^bACE2: angiotensin-converting enzyme 2.

^cKD app: apparent dissociation constant.

^dA positive Δlog10 KD app value relative to the unmutated SARS-CoV-2 receptor-binding domain (3.9 × 10⁻¹¹ M) indicates stronger binding.

^eMFI: mean fluorescence intensity.

^fPositive Δlog MFI values relative to the unmutated SARS-CoV-2 receptor-binding domain indicate increased expression.

^gRSA: relative solvent accessibility.