Figure 1.

Schematic of eIVFG procedure and examples of endpoint analyses. Dissected ovaries are broken down mechanically or enzymatically to isolate follicles. These follicles then undergo a recovery incubation prior to the selection of high-quality follicles. Follicles are encapsulated in alginate beads individually (multilayer secondary follicles) or in cohorts of 10 (two-layer early secondary follicles) and then undergo another recovery incubation. Alginate-encapsulated follicles are then plated in a 96-well plate and cultured. Imaging and media changes take place every other day throughout the duration of the culture, and various endpoint parameters can be assessed.