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Preparation of follicles for histology. (A) Follicles are re-encapsulated in alginate beads and stained with Alcian blue after fixing with 3.8% PFA. The stained beads can then be dehydrated and processed for embedding. (B) Blue-stained bead should be visible within the paraffin after embedding. (C) H&E-stained section containing follicles.
