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. 2022 Sep 22;108(1):5–22. doi: 10.1093/biolre/ioac176

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Published by Oxford University Press on behalf of Society for the Study of Reproduction 2022.

This work is written by US Government employees and is in the public domain in the US.

PMC Copyright notice

Assessment of follicle survival. (A) Examples of dead follicles. (B) Examples of two-layer secondary follicle cultures with high survival (top) and low survival (bottom). High survival follicles are characterized by an even border, continuous growth and translucent granulosa cell layers until the antrum is formed. Low survival follicles characteristics include granular appearance, opaque granulosa cells prior to antrum formation, uneven borders, and low growth rates. (C) Example of survival curves for cultures with high and low survival. (D) Examples of follicles that have a partially extruded oocyte and then recover later in culture. D0, culture day 0; D6, culture day 6; D8, culture day 8; D10, culture day 10.