Table 3.
Materials, consumables, and equipment used for eIVFG
| Equipment | Example | |
|---|---|---|
| Class II biosafety cabinet | Baker SterilGARD® III Advance Safety Cabinets SKU: 8038-30-1044 | |
| CO2 incubator | Heracell VIOS 160i CO₂ Incubator, Thermo Scientific VWR # 76459-168 | |
| Dissection microscope | Leica; MZ9.5, S9 series | |
| Heat stage | Tokai-Hit; Thermoplate TPI series heated glass stage | |
| Laminar flow hood | Cole-Parmer; Vertical Laminar Flow Cabinet #UX-78902-40 | |
| IVF cabinet (Encompasses dissecting scope, heated stage, and laminar flow hood) | IVFtech; Classica workstation | |
| Inverted imaging platform for culture plates | EVOS; ThermoFisher Scientific, AMF5000 | |
| Material | Source | Catalog no |
| Watch glass (45 mm) | Electron Microscopy Sciences | 7054345 |
| 28 ½ G insulin syringe | Exelint International, Co. | 26027 |
| Nunc IVF dish | ThermoScientific | 150260 |
| 35 mm Petri dish | Corning | 351008 |
| Disposable scalpel | Fisher Scientific | 12-460-453 |
| Low retention pipette tips | MIDSCI | PR-10RK-NS |
| Stripper/mouth pipette | Origio/homemade | MXL3-STR |
| Stripper tips (125 μm) | Origio | MXL3-125 |
| Stripper tips (150 μm) | Origio | MXL3-150 |
| 96-well culture plates | Corning | 3595 |