Table 4.
List of tools, dishes, and media needed for each step of eIVFG
| Step | Materials | Timing |
|---|---|---|
| Ovary dissection | 35 mm Petri dish with DM Fine tweezers or insulin needles | ~5–10 min |
| Isolation | Stripper or mouth pipette with stripper tips | ≤45 min |
| Mechanical isolation | 45 mm watch glass with DM | |
| 2 insulin syringes | ||
| Or | IVF dish with DM | |
| Enzymatic isolation | Scalpel | |
| 35 mm Petri dish with EM | ||
| IVF dish with DM | ||
| Heated shaker | ||
| Recovery incubation | IVF dish with MM (equilibrated) | 1 h |
| Selection | Stripper or mouth pipette with stripper tips IVF dish with DM | 1 h |
| Encapsulation | Stripper or mouth pipette with stripper tips | 15–45 min |
| Curved forceps | Depends on number of follicles | |
| Drop method | 35 mm Petri dish lid | |
| 10 μL displacement pipette | ||
| P10 low retention pipette tips | ||
| Or | 35 mm Petri dish with calcium solution | |
| 35 mm Petri dish with DM | ||
| Parafilm method | 35 mm Petri dish lid | |
| Parafilm cut into 0.5–1 cm2 squares | ||
| 35 mm Petri dish with calcium solution | ||
| 35 mm Petri dish with DM | ||
| Recovery incubation | 35 mm Petri dish with MM (equilibrated) | 45 min |
| Plating | Flat bottom 96-well plate with GM (equilibrated) Curved forceps | <15 min |