Fig. 3.

Effects of Naticol^Ⓡ on skin melanogenesis-related factors, melanin contents (10˟) (A), tyrosinase activity (B), nitric oxide (C), cAMP (D), glutathione (E), p-PKA (F), p-CREB (G), MITF (H), TRP-1 (I), and TRP-2 (J), and protein band (K) in IBMX-irradiated B16F10 cells. The cells were treated with 250 µM IBMX, except NC, and incubated for 24 h with 100 µg/mL of arbutin (PC) and various concentrations (100, 200, and 400 µg/mL) of Naticol^Ⓡ collagen. Values are presented as mean±SD. Different letters (a-f) represent significant differences at P<0.05, as determined by Duncan’s multiple range test. The biomarkers used for Western blot analysis are listed in Table 1. NC, normal control; C, control; PC, positive control; UVB, ultraviolet B; IBMX, 3-isobutyl-1-methylxanthine.