Table 2.

Primer sets used for real-time polymerase chain reaction

Gene	Sequence (5’→3’)
HAS1 (M)
Forward	TCA GGG AGT GGG ATT GTA GGA
Reverse	AAA TAG CAA CAG GGA GAA AAT GGA
HAS2 (M)
Forward	AAT ACA CGG CTC GGT CCA AGT
Reverse	CCA TCG GGT CTG CTG GTT
HAS3 (M)
Forward	GGC CAT GGG AGC TAA AGT TG
Reverse	CCA AAT TGA TGT TGA AAC TCT TGA AA
LCB1(SPT) (M)
Forward	AGC GCC TGG CAA AGT TTA TG
Reverse	GTG GAG AAG CCG TAC GTG TAA AT
DEGS1 (M)
Forward	CCG GCG CAA GGA GAT CT
Reverse	TGT GGT CAG GTT TCA TCA AGG A
Fibrillin-1 (M)
Forward	ACA ATT GTT CAC CGA GTC GAT CT
Reverse	ACT GTA CCT GGG TGT TGC CAT T
TGF-β RI (M)
Forward	CAT CCT GAT GGC AAG AGC TAC A
Reverse	TAG TGG ATG CGG ACG TAA CCA
Procollagen type I (M)
Forward	TTA CGT GGC AAG TGA GGG TTT
Reverse	TGT CCA GAT GCA CTT CTT GTT TG
Collagen type I (M)
Forward	GAC CGT TCT ATT CCT CAG TGC AA
Reverse	CCC GGT GAC ACA CAA AGA CA
GAPDH (M)
Forward	CAT GGC CTT CCG TGT TCC TA
Reverse	GCG GCA CGT CAG ATC CA
HAS2 (H)
Forward	GAA ACA GCC CCA GCC AAA
Reverse	AAG ACT CAG CAG AAC CCA GGA A
LCB1(SPT) (H)
Forward	CCA TGG AGT GGC CTG AAA GA
Reverse	CTG ACA CCA TTT GGT AAC AAT CCT A
DEGS1 (H)
Forward	GCT GAT GGC GTC GAT GTA GA
Reverse	TGA AAG CGG TAC AGA AGA ACC A
Elastin (H)
Forward	GTC GGA GTC GGA GGT ATC
Reverse	TGA GAA GAG CAA ACT GGG
TGF-β R1 (H)
Forward	TCC CGG CAG ATC AAC GA
Reverse	ACG CGG TCA CAA ACA TGG T
Procollagen (H)
Forward	TCT CCT CCG AAG GGA ATG AAC
Reverse	CAG CGG TGA CAC TGA GAT CTG
Collagen type Ⅰ (H)
Forward	GCC TCG GAG GAA ACT TTG C
Reverse	TCC GGT TGA TTT CTC ATC ATA GC
GAPDH (H)
Forward	CCC CAC ACA CAT GCA CTT ACC
Reverse	TTG CCA AGT TGC CTG TCC TT

M, mouse; H, human; HAS, hyaluronic acid synthase; LCB1(SPT), long chain base biosynthesis protein 1 (serine palmitoyltransferase); DEGS1, delta 4-desaturase sphingolipid 1; TGF-β RI, transforming growth factor beta receptor 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.