Figure 4. Molecular genetic analysis of the CIC-sarcoma. (A) Gel electrophoresis of reverse transcription (RT) PCR amplification products: lane 1, amplification of a 300 bp cDNA fragment using the forward primer CIC-4377F and the reverse primer DUXL4-1553R1. M, GeneRuler 1 kb Plus DNA ladder (ThermoFisher Scientific). (B) Partial sequence chromatogram showing the junction in the CIC::DUX4 fusion transcript type 1. (C) Partial sequence chromatogram showing the junction in the CIC::DUX4 fusion transcript type 2. The triplets and the corresponding coding amino acids are shown. Stop codon is noted with ***. The common four nucleotides AGGT at the junctions are underlined.