FIG. 3.
(A) Mouse IgG F(ab′)2 fragments inhibit the binding of SfbI to human IgG Fc. The binding of SfbI to human IgG Fc was performed in the presence of increasing concentrations of either mouse or human IgG F(ab′)2 fragments. The values are means of three determinations; one representative out of three independent experiments is shown. Standard deviations were lower than 10%. (B) Schematic representation of the different domains of the SfbI protein. (C) Identification of the SfbI domain able to bind to the F(ab′)2 fragment of mouse IgG. Results are the averages of three independent determinations. Standard deviations are indicated by vertical lines.