FIG. 4.

(A) Stimulation of human PBLs and mouse spleen cells by SfbI. The values are calculated as follows: mean counts per minute of SfbI-stimulated cells − mean counts per minute in the absence of SfbI. Shown are the means ± standard errors of the means of triplicate samples. (B) Binding of SfbI to mouse spleen cell subsets. Splenocytes were incubated with SfbI, double stained using antibodies specific for SfbI and surface cellular markers, and analyzed by flow cytometry. The mean fluorescence intensity (MFI) index was calculated as follows: MFI value in the presence of SfbI/MFI value in the absence of SfbI.