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. 2023 Jan 16;24:25. doi: 10.1186/s12864-023-09113-x

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Genome-wide association analysis of parapodium number. a Manhattan chart display of GWAS results, Only the most significant 100 SNP loci located in Scaffold are shown in the figure. b QQ plot of GWAS results. c Comparative analysis of parapodium number between two subgroups using a t-test (d, e) Two LD blocks related to parapodium number. f Results of qRT-PCR validation of genes (** means P < 0.01 in t-test. * means P < 0.05 in t-test)