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. 2023 Jan 16;21:28. doi: 10.1186/s12967-023-03883-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — EREG proliferation analysis. A Viability of cells (U251) after treatment with elesclomol with or without 10 mM of indicated metals. B V iability of U251 cells was assessed at the indicated times after elesclomol-Cu (1:1 ratio) treatment. ES, elesclomol. (C) Viability of shNC and shEREG in U251 cells was assessed at the indicated times after elesclomol-Cu (1:1 ratio) treatment. ES, elesclomol. D Cell viability of U251 cells after knocking down EREG was determined using CCK8 assays with or without the presence of 20 nM elesclomol-Cu. E U87 and U251 cells were treated with EdU for 6 h prior to click reaction. Data analysis was performed to calculate the signal intensity in EdU-positive cells based on individual DAPI signal and is displayed in the right graph. F Cell cycle distribution was analyzed by PI staining in U251 and U87 cells of shNC and shEREG. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the mean ± SD