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. 2023 Jan 16;23:7. doi: 10.1186/s12935-022-02836-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 10 — Validation of the expression levels of CRGs in cells and tissues. A Comparison of the expression levels of 10 CRGs in pancreatic cancer cell lines and normal cells using qRT‒PCR. *P < 0.05, **P < 0.01, ***P < 0.001. B Comparison of the expression levels of DLAT in PAAD tissues and adjacent nontumorous tissues (ANT) via IHC (scale bar: 100 μm). C Western blot analysis of DLAT protein levels; GAPDH was used as a loading control. D IHC staining indicated that DLAT was overexpressed in PAAD tissues (clinical stages I–IV) compared to in normal pancreatic tissues (scale bar: 100 μm). E Kaplan‒Meier curves of PAAD patients with low versus high expression levels of DLAT. F, G Forest plots for univariate (F) and multivariate (G) Cox regression analyses of the prognostic value